a)
The given data is as follows,
Comment Step 2 of 7
The saturation or mm polot between [S] and [v] is as folows,
The plot of v versus S indicates that it as a
reaction. As the action of enzyme got inhibited with the increase in the concentration of the substrate.Comment Step 3 of 7
b)
Determining the constants
: To determine 
, 
and 
are are plotted to obtain a Line-WeaverBurk plot, as given below:
Comment Step 4 of 7
and 
are determined from low substrate concentration, where inhibition is not effective.
From the obtained LB plot the line of equation is
.Calculation of
is as follows,
Thus, the
Comments (4) Step 5 of 7
The calculation of
is as follows,
Thus, the
Comments (3) Step 6 of 7
Determining
from maximum reaction rate: which can be determined by setting ,
From plot,
90 mg/l
Therefore,
Comments (1) Step 7 of 7
c)
Rate of reaction at [S] = 70 mg/l, Since inhibition effect is not observed at this substrate, the equation 3.35 must be used
Therefore, the oxidation rate at [S] = 70 mg/l is
Comment
